Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded by the yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of the ribosome
Identifieur interne : 000224 ( France/Analysis ); précédent : 000223; suivant : 000225Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded by the yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of the ribosome
Auteurs : M. Zagulski [Pologne] ; D. Kressler [Suisse] ; A.-M. Bécam [France] ; J. Rytka [Pologne] ; C. J. Herbert [France]Source :
- Molecular Genetics and Genomics [ 1617-4615 ] ; 2003-11-01.
English descriptors
Abstract
Abstract: In this study, we show that the Saccharomyces cerevisiae ORF YBR142w, which encodes a putative DEAD-box RNA helicase, corresponds to MAK5. The mak5-1 allele is deficient in the maintenance of the M1 dsRNA virus, resulting in a killer minus phenotype. This allele carries two mutations, G218D in the conserved ATPase A-motif and P618S in a non-conserved region. We have separated these mutations and shown that it is the G218D mutation that is responsible for the killer minus phenotype. Mak5p is an essential nucleolar protein; depletion of the protein leads to a reduction in the level of 60S ribosomal subunits, the appearance of half-mer polysomes, and a delay in production of the mature 25S and 5.8S rRNAs. Thus, Mak5p is involved in the biogenesis of 60S ribosomal subunits.
Url:
DOI: 10.1007/s00438-003-0913-4
Affiliations:
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ISTEX:7AB379BE0C3D8711416C866FB9C09828861E074ALe document en format XML
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Bécam</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire propre du CNRS associé à l'Université Pierre et Marie Curie, Centre de Génétique Moléculaire, 91198, Gif-sur-Yvette</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Gif-sur-Yvette</settlement></placeName></affiliation></author><author><name sortKey="Rytka, J" sort="Rytka, J" uniqKey="Rytka J" first="J." last="Rytka">J. Rytka</name><affiliation wicri:level="1"><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, 5a Pawinskiego, 02-106, Warsaw</wicri:regionArea><wicri:noRegion>Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Herbert, C J" sort="Herbert, C J" uniqKey="Herbert C" first="C. J." last="Herbert">C. J. Herbert</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire propre du CNRS associé à l'Université Pierre et Marie Curie, Centre de Génétique Moléculaire, 91198, Gif-sur-Yvette</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Gif-sur-Yvette</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Genetics and Genomics</title><title level="j" type="abbrev">Mol Genet Genomics</title><idno type="ISSN">1617-4615</idno><idno type="eISSN">1617-4623</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2003-11-01">2003-11-01</date><biblScope unit="volume">270</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="216">216</biblScope><biblScope unit="page" to="224">224</biblScope></imprint><idno type="ISSN">1617-4615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1617-4615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DEAD-box helicase</term><term>M1 dsRNA virus</term><term>MAK5</term><term>Ribosome biogenesis</term><term>Saccharomyces cerevisiae</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In this study, we show that the Saccharomyces cerevisiae ORF YBR142w, which encodes a putative DEAD-box RNA helicase, corresponds to MAK5. The mak5-1 allele is deficient in the maintenance of the M1 dsRNA virus, resulting in a killer minus phenotype. This allele carries two mutations, G218D in the conserved ATPase A-motif and P618S in a non-conserved region. We have separated these mutations and shown that it is the G218D mutation that is responsible for the killer minus phenotype. Mak5p is an essential nucleolar protein; depletion of the protein leads to a reduction in the level of 60S ribosomal subunits, the appearance of half-mer polysomes, and a delay in production of the mature 25S and 5.8S rRNAs. Thus, Mak5p is involved in the biogenesis of 60S ribosomal subunits.</div></front></TEI><affiliations><list><country><li>France</li><li>Pologne</li><li>Suisse</li></country><region><li>Île-de-France</li></region><settlement><li>Gif-sur-Yvette</li></settlement></list><tree><country name="Pologne"><noRegion><name sortKey="Zagulski, M" sort="Zagulski, M" uniqKey="Zagulski M" first="M." last="Zagulski">M. Zagulski</name></noRegion><name sortKey="Rytka, J" sort="Rytka, J" uniqKey="Rytka J" first="J." last="Rytka">J. Rytka</name></country><country name="Suisse"><noRegion><name sortKey="Kressler, D" sort="Kressler, D" uniqKey="Kressler D" first="D." last="Kressler">D. Kressler</name></noRegion></country><country name="France"><region name="Île-de-France"><name sortKey="Becam, A M" sort="Becam, A M" uniqKey="Becam A" first="A.-M." last="Bécam">A.-M. Bécam</name></region><name sortKey="Herbert, C J" sort="Herbert, C J" uniqKey="Herbert C" first="C. J." last="Herbert">C. J. Herbert</name><name sortKey="Herbert, C J" sort="Herbert, C J" uniqKey="Herbert C" first="C. J." last="Herbert">C. J. Herbert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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